Shoot and root formation obtained on selected species of the Gesneriaceae through tissue culture.

Author(s): 
Beaufort-Murphy, H.T.
Year: 
1984
Journal: 
Selbyana
Volume: 
7
Issue: 
2–4
Page Range: 
274–280
Keywords: 
Root, Shoot, Gesneriaceae, Tissue-culture, Taxonomy
Abstract: 
Growth responses of leaf explants in sterile culture were studied for ten taxa chosen from both subfamilies to represent the wide structural diversity of the Gesneriaceae: Chirita sinensis Lindl., Streptocarpus saxorum Engl., Streptocarpus 'Constant Nymph,' Saintpaulia ionantha Wendland, in the Cyrtandroideae; and Koellikeria erinoides (DC.) Mansf., x Koellikohleria rosea (Lehm.) H.E. Moore, Sinningia speciosa (Lodd.) Hiern, and Sinningia tubiflora (Hook.) Fritsch in the Gesnerioideae. An array of 26 combinations of kinetin, indoleacetic acid, naphthaleneacetic acis, and coconut milk in agar media was used; plant responses in terms of shoot, root, and callus production were recorded. Initiation of numerous vigorous shoots occurred in Chirita sinensis, Streptocarpus 'Constant Nymph,' Koellikeria erinoides, and Sinningia speciosa. After subculture on rooting medium, plants of these four taxa were potted and grown to maturity. Shoot initiation also occurred in Streptocarpus saxorum and Saintpaulia ionantha but the shoots were few and poor in vigor. Implications of the results for tissue-culture propagation of Gesneriaceae are discussed as possible correlations between culture responses and taxonomic relationships within the family.